Quality

The method used for concentrating stem cells has a direct impact on the stem cells. In order to deliver enough progenitor cells, the stem cells not only need to be concentrated but concentrated in such a way as to leave them viable and functional. Stem cells concentrated using the SmartPReP BMAC system meet these critical criteria.

When concentrated BMAC stem cells and commercially available hMSC's (used as a control) are exposed to Mesenchymal Stem Cell culture media, the BMAC culture demonstrated a retained capacity for cell division and maintain normal morphology when compared to the control hMSC cells.

When concentrated BMAC stem cells and commercially available hMSC's are exposed to an osteogenic differentiation medium (ODM), the BMAC cells and control cells retained their ability and potential for osteoblastic differentiation.

Bright areas represent new deposition elements

Finally, when concentrated BMAC stem cells are exposed to Von Kossa silver stains, the BMAC cells demonstrated osteoblastic differentiation as evidenced by the vacuoles with black stain indicating deposited calcium.