Performance

There has been a lot of excitement regarding the use of bone marrow derived stem cells; especially in orthopedics as a potential to eliminate the complications associated with iliac crest graft harvesting. Consequently, it is critical to choose a technology platform that is not only documented to reliably concentrate all the stem and accessory cells but also concentrates these cells without diminishing their biologic potential.

As an example, selective retention techniques are less than optimal processing systems. The process routinely fails to capture a large number of cells present in the bone marrow aspirate. Furthermore, the end product is limited in the types of cells, molecules and proteins it is able to recover from bone marrow and return to the patient.

Unlike these selective retention techniques, the SmartPReP BMAC system is designed to concentrate a buffy coat from whole bone marrow aspirate. Because a buffy coat is a physiological fraction, it contains the total compliment of all stem cell types as well as platelets and other soluble factors that play a role in skeletalgenesis.

Data on File

Bone Marrow Aspirate Vol., ml

Total Nucleated Cells
x10⁶/ml

Platelets
x10⁶/ml

Mononuclear Cells
x10⁶/ml

CD34+
x10³/ml

* Colony Forming Units-F/cm³

Total Progenitor Cells

Selective Retention

35.6

126.6

9.6

412

841

7,575

BMAC

120

197.2

1,182.6

43.8

1,384

2,662

50,596

* Hernigou, et al, (Percutaneous Autologous Bone Marrow Grafting - Influence of the Number and Concentration of Stem Cells, JBJS), showed healing of nonunions in patients receiving > 1,500 CFU-F/cm³