Process Purified, Adipose Tissue Into a Purified MSC-Rich Graft in Just 4 Minutes1
The quality of adipose graft material is an important consideration prior to performing any lipograft procedure. A major challenge in autologous lipografting is graft volume loss, which can greatly affect aesthetic outcomes. Resorption of fluid, oils and lipids contributes to graft volume loss; in addition, these materials can be toxic to the cellular components of the graft.20,22,24,27
The Harvest® AdiPrep® Adipose Concentration System concentrates tissue samples to deliver a graft with high stem cell and nucleated cell counts while significantly reducing excess fluids that contribute to graft volume loss. The resulting purified adipose concentrate can then be used for clinical grafting procedures, such as facial contouring or musculoskeletal regenerative applications.
Processing of lipoaspirate with the Harvest AdiPrep system provides several critical advantages, such as:
- Removes excess infranatant fluid, minimizing graft resorption and the need to overfill20
- Removes excess oils, lipids and cellular debris that may induce an inflammatory response in patients19,20
- Retains and concentrates the tissue stromal vascular fraction (SVF), which includes all of the cell types and the structural matrix needed to promote graft survival and the cellular complement needed to aid in tissue regeneration19,21,22
Removal of Excess Infranatant Fluid
Excess infranatant fluid in a graft sample makes it challenging to estimate an accurate graft volume for a procedure, because this fluid is resorbed by the body. In an attempt to account for resorption, physicians typically overfill the treatment site with lipoaspirate, resulting in increased swelling and an undesirable initial aesthetic result.24,27 Removal of excess fluid can minimize the need for overfilling and offer a more predictable aesthetic outcome.20
Removal of Excess Oils and Lipids
Oils, lipids, and cellular debris that are present in lipoaspirate may cause an inflammatory response that prolongs graft healing and can prove toxic to the cellular components of the graft itself. Removal of these materials may promote graft survival.20,24
Retention of the Tissue Stromal Vascular Fraction (SVF)
Tissue SVF contains the total adipose tissue microenvironment, including a variety of cell types, such as adipocytes, pre-adipocytes and mesenchymal stem cells (MSCs). Tissue SVF also contains a structural matrix to which cells can attach; this matrix supports cell viability and proliferation and promotes graft retention.20
The Harvest AdiPrep System Versus Gravity Decantation
Gravity decantation, the most common technique for processing a sample of lipoaspirate, does not remove lipids and excess fluids effectively and may produce a poor-quality graft. The Harvest AdiPrep system isolates oils and lipids using a proprietary lipid barrier disc technology built into the Harvest AdiPrep Process Disposable.
The chart below shows the separation quality in final processed adipose tissue samples using standard gravity decantation versus the Harvest AdiPrep system. See the difference for yourself.